Estudio de la citotoxicidad de puntos de carbono dopados con Gd3+

Baquero Castillo, Karol Julissa | 2022

The cytotoxic effect of gadolinium ion-doped carbon dots being studied as a potential radiosensitizing agent to improve the radiotherapy performance in cancer treatment is characterized using radioresistant cancer cells as a model. The MTT assay is one of the most common assays for cytotoxicity testing, including its practicality in the laboratory. This colorimetric test allows the metabolic effect and cell viability to be studied by exposing the cells under study to different treatments. The MTT reagent is metabolized by dehydrogenases, mainly in the mitochondria and formazan molecules are formed, with a purple coloration. They are stored inside the living cells due to their insolubility. This project studies the cytotoxic effect of undoped (PCs) and gadolinium ion-doped (PC: Gd) carbon dots in the primary cell culture of a glial tumor patient and the VERO cell line by MTT assay. The synthesis and characteristics of the nanoparticles are presented, followed by cytotoxicity tests to evaluate the cytotoxic effect on tumor cells and healthy cells. Finally, the development of the statistical analysis allows acquiring a tool to support the results obtained by performing this cytotoxicity test and to determine the number of tests that got a strong statistical potential to validate the results of the cytotoxic effect of the carbon dots studied.